Optimized technologies of large-scale cryopreservation of cyprinid fish sperm
https://doi.org/10.37663/0131-6184-2022-6-58-66
Abstract
The need for optimization of technologies of fish sperm cryopreservation is conditioned by instability of fresh sperm quality. Presented protocols of cyprinids sperm cryopreservation have been optimized following the results of experiments carried out on varied-quality biomaterial. These protocols ensure with high effectiveness both freezing big volumes of sperm under laboratory and field conditions. Effectiveness of the protocol with two-stage freezing regime in addition has been confirmed by results of cryopreservation of cyprinids sperm samples for addition to the collection of low-temperature gene bank of VNIIPRKH for the last four years.
About the Authors
O. B. Dokina
Laboratory of Cryobiology of the Freshwater Fisheries Branch of the VNIRO Federal State Budgetary Institution ("VNIIPRH")
Russian Federation
Russian Federation
O.B. Dokina – Candidate of Chemical Sciences, Leading Researcher
K. V. Kovalev
Laboratory of Cryobiology of the Freshwater Fisheries Branch of the VNIRO Federal State Budgetary Institution ("VNIIPRH")
Russian Federation
Russian Federation
K.V. Kovalev – Candidate of Agricultural Sciences, Head of the laboratory
N. D. Pronina
Laboratory of Cryobiology of the Freshwater Fisheries Branch of the VNIRO Federal State Budgetary Institution ("VNIIPRH")
Russian Federation
Russian Federation
N.D. Pronina – Chief Specialist
V. A. Milenko
Laboratory of Cryobiology of the Freshwater Fisheries Branch of the VNIRO Federal State Budgetary Institution ("VNIIPRH")
Russian Federation
Russian Federation
V.A. Milenko – Specialist
V. N. Kovalenko
Dmitrov Fisheries Technological Institute (branch) of the AGTU
Russian Federation
Russian Federation
V.N. Kovalenko – Associate Professor of the Department of Food Technology and Refrigeration Technology
References
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Review
For citations:
Dokina O.B., Kovalev K.V., Pronina N.D., Milenko V.A., Kovalenko V.N. Optimized technologies of large-scale cryopreservation of cyprinid fish sperm. Fisheries. 2022;(6):58-66. (In Russ.) https://doi.org/10.37663/0131-6184-2022-6-58-66
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