Optimized technology of large-scale cryopreservation of sturgeon sperm
https://doi.org/10.36038/0131-6184-2025-3-147-160
EDN: ZYCETV
Abstract
To preserve the genetic diversity of sturgeon during artificial reproduction, the use of cryopreserved sperm samples stored in cryobanks is becoming increasingly important. The observed trend towards a decrease in the quality of seed material from fish farms determines the need to improve cryopreservation technologies. Based on the results of experiments conducted over six years on ejaculates of varying quality, the basic technology for cryopreservation of sturgeon sperm that we had previously developed was optimized. The use of optimized technology will allow for the highly efficient freezing of large volumes of sperm for reproductive and biodiversity conservation purposes.
Supporting agencies: The work was supported by a grant from the Ministry of Science and Higher Education of the Russian Federation 075-15-2021-1084 for the development of genetic technologies.
About the Authors
O. B. Dokina
Branch for Freshwater Fisheries of «VNIRO» («VNIIPRH»)
Russian Federation
Russian Federation
Olga B. Dokina – Candidate of Chemical Sciences, Leading Researcher at the Laboratory of Cryobiology
Moscow region, Dmitrovsky city district, 141821, village Rybnoye, 40A
K. V. Kovalev
Branch for Freshwater Fisheries of «VNIRO» («VNIIPRH»)
Russian Federation
Russian Federation
Konstantin V. Kovalev – Candidate of Agricultural Sciences, Head of the Cryobiology Laboratory
Moscow region, Dmitrovsky city district, 141821, village Rybnoye, 40A
N. D. Pronina
Branch for Freshwater Fisheries of «VNIRO» («VNIIPRH»)
Russian Federation
Russian Federation
Natalia D. Pronina – chief Specialist of the Cryobiology Laboratory
Moscow region, Dmitrovsky city district, 141821, village Rybnoye, 40A
D. A. Popov
Branch for Freshwater Fisheries of «VNIRO» («VNIIPRH»)
Russian Federation
Russian Federation
Dmitry A. Popov – Specialist of the Cryobiology Laboratory
Moscow region, Dmitrovsky city district, 141821, village Rybnoye, 40A
O. V. Korabelnikova
Branch for Freshwater Fisheries of «VNIRO» («VNIIPRH»)
Russian Federation
Russian Federation
Olga V. Korabelnikova – Candidate of Biological Sciences, leading Specialist of the Cryobiology Laboratory
Moscow region, Dmitrovsky city district, 141821, village Rybnoye, 40A
V. N. Kovalenko
Dmitrov Fish-industry Technological Institute (Branch) of «ASTU»
Russian Federation
Russian Federation
Vladimir N. Kovalenko – Associate Professor of the Department of Food Technology and Refrigeration
Moscow region, Dmitrovsky city district, 141821, village Rybnoye, 36
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Review
For citations:
Dokina O.B., Kovalev K.V., Pronina N.D., Popov D.A., Korabelnikova O.V., Kovalenko V.N. Optimized technology of large-scale cryopreservation of sturgeon sperm. Fisheries. 2025;(3):147-160. (In Russ.) https://doi.org/10.36038/0131-6184-2025-3-147-160. EDN: ZYCETV
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