Method of express determination of the number of microalgae cultures of the genus Tetraselmis
https://doi.org/10.37663/0131-6184-2022-4-76-80
Abstract
The article presents an algorithm for using spectrophotometric measurement to determine the number of cells of cultures of microalgae of the genus Tetraselmis filtered and unfiltered samples. The dependence "density – number of cells" is approximated by a linear equation with a high correlation coefficient, more than 75 %, which confirms the high reliability of the method. The ability to measure the density of unfiltered culture samples on a spectrophotometer allows you to quickly estimate the number of cells both in the field and on an industrial scale.
Supporting agencies: The work was financially supported by a grant from the Russian Science Foundation (project No. 21-74-30004)
About the Authors
L. A. Botsun
Laboratory of Marine Ecosystem Dynamics
Russian Federation
Russian Federation
PhD student, Lead Engineer
Marine Ecosystem Dynamics Laboratory
Vladivostok
Zh. V. Markina
Laboratory of Marine Ecosystem Dynamics
Russian Federation
Russian Federation
Candidate of Biological Sciences, Researcher, Researcher at the Laboratory
Laboratory of Cellular Technologies
Vladivostok
S. I. Maslennikov
Laboratory of Marine Ecosystem Dynamics
Russian Federation
Russian Federation
Senior Researcher
Laboratory of Marine Ecosystem Dynamics
Vladivostok
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Review
For citations:
Botsun L.A., Markina Zh.V., Maslennikov S.I. Method of express determination of the number of microalgae cultures of the genus Tetraselmis. Fisheries. 2022;(4):76-80. (In Russ.) https://doi.org/10.37663/0131-6184-2022-4-76-80
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