Effectiveness of the use of saline solution in artificial insemination of caviar of rainbow trout Oncorhynchus mykiss (Waibaum, 1972) and Ladoga palia Salvelinus alpinus lepechini (Gmelin, 1788)
https://doi.org/10.37663/0131-6184-2023-2-96-100
Abstract
In order to increase the level of fertilization of eggs, the effect of buffer solution on sperm activity and the effectiveness of its use as a medium for insemination eggs of rainbow trout which was grown in conditions of flowing water and in recirculation aquaculture system (RAS), as well as Ladoga palia, were tested. In male trout and palia, the number of motile spermatozoa and their activity were higher in solution than in water. In trout which was grown in running water, the percentage of eggs fertilization increased significantly when use solution only in cases when the eggs were contaminated with the contents of burst eggs. In fish grown in the RAS, the replacement of water with a buffer solution increased the level of fertilization in all test variants, regardless of the presence or absence of contamination of the eggs. In Ladoga palia, the result of insemination was the same in water and in solution.
About the Authors
N. I. Shindavina
Federal Breeding and Genetic Center of Fish Farming (FSGCR branch of FSBI "Glavrybvod")
Russian Federation
Russian Federation
N.I. Shindavina – Candidate of Biological Sciences, Leading researcher of the Fish Breeding Laboratory
village Ropsha, Leningrad region
A. G. Moseev
Federal Breeding and Genetic Center of Fish Farming (FSGCR branch of FSBI "Glavrybvod")
Russian Federation
Russian Federation
A.G. Moseev – Head of the Fish Breeding site
village Ropsha, Leningrad region
V. Ya. Nikandrov
Federal Breeding and Genetic Center of Fish Farming (FSGCR branch of FSBI "Glavrybvod")
Russian Federation
Russian Federation
V.Ya. Nikandrov – Candidate of Biological Sciences, leading researcher of the Fish Breeding Laboratory
village Ropsha, Leningrad region
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For citations:
Shindavina N.I., Moseev A.G., Nikandrov V.Ya. Effectiveness of the use of saline solution in artificial insemination of caviar of rainbow trout Oncorhynchus mykiss (Waibaum, 1972) and Ladoga palia Salvelinus alpinus lepechini (Gmelin, 1788). Fisheries. 2023;(2):96-100. (In Russ.) https://doi.org/10.37663/0131-6184-2023-2-96-100
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